The New Genetic Engineering Toolbox

Peering into the Natural Genetic Engineering Toolbox

Peering into the Natural Genetic ligate themselves to DNA also mediate the relatively new Engineering Toolbox phenomenon of rolling-circle transposition, as illustrated by the bacterial IS91 element (M Pilar Garcillá n-Barcia et al.). Rolling circle transposition also occurs in Mobile DNA II eukaryotes and is significant because it generates the The amazing and rapidly growing family of rearran...

New and TALENted genome engineering toolbox.

Recent advances in the burgeoning field of genome engineering are accelerating the realization of personalized therapeutics for cardiovascular disease. In the postgenomic era, sequence-specific gene-editing tools enable the functional analysis of genetic alterations implicated in disease. In partnership with high-throughput model systems, efficient gene manipulation provides an increasingly pow...

Metabolic engineering--a genetic toolbox for small molecule organic synthesis.

Metabolic engineering is an emerging field that exploits biosynthetic machinery as a means to genetically design small molecule production within heterologous host organisms. Molecular design and synthesis with biological tools has lagged behind total synthesis technology for about seventy-five years and owes its existence to relatively new molecular biology techniques. Here the field of metabo...

The MATLAB Genetic Algorithm Toolbox

Genetic algorithms (GAs) are stochastic global search and optimization methods that mimic the metaphor of natural biological evolution [1]. GAs operate on a population of potential solutions applying the principle of survival of the fittest to produce successively better approximations to a solution. At each generation of a GA, a new set of approximations is created by the process of selecting ...

Algorithm Engineering for the Basic Toolbox